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THERMAL CYCLERS (PCR Technology):
The reliability of a PCR results is dependent on its sensitivity, specificity and reproducibility. An essential prerequisite for a successful PCR is efficient sample preparation, as well as thermal cyclers with excellent temperature precision and block
 
homogeneity. Apart from well defined dedicated PCR step-up, well trained staff, the recent technological advances in thermal cyclers from Applied Biosystems and MJ Research has been successfully adapted at SRL to optimize PCR assays with explicit sensitivity and specificity. Not just simple PCR but all the hyphenated forms of PCR i.e. PCR-RFLP, Nested PCR, Multiplex PCR, ARMS PCR etc. have been successfully standardized and utilized for diagnosing range of infectious diseases, genetic disorders,
cancer etc.
 
 
Real Time PCR:
First Laboratory in India to offer Real Time Technology for quantitative monitoring of HIV, HBV and HCV Viral Loads. The development of real-time quantitative PCR has eliminated the variability traditionally associated with quantitative PCR, thus allowing the routine and reliable
 
quantification of PCR products. This technology therefore, now provides means of accurate, sensitive and reproducible measurements of viral loads directly in clinical specimens. Apart from this the technology is effectively used in other applications such as gene expression studies, mutation detection, allelic discrimination and optimizing PCR conditions. Advanced oncology markers for minimal residue disease detection have been successfully developed and implicated for monitoring purpose. At SRL Real Time PCR platforms from Corbett Research and Stratagene are effectively used for development as well as testing purpose.
 
 
GENETIC DNA ANALYSER (Sequencer): The sequencing platform available at SRL is a state of art Genetic analyzer facility from Applied Biosystems that has been designed to maximize capacity while maintaining efficiency, scalability and flexibility of both research and clinical applications. The platform is well recognized internationally for conducting advanced tests such as HIV Genotyping  
and Drug Resistance. Apart from mutation detection, genotyping analysis for Molecular diagnostics applications, other super specialized analysis i.e. SNP analysis, Fragment analysis, QFPCR etc. are performed for the R&D scientists studying molecular mechanisms for various diseases and disorders such as cancer, diabetes, asthma , and others. The accuracy and quality of the sequencing data generated every day is monitored through the stringent and regular QC/QA protocols and checks.
 
 
FISH:
Fluorescence in situ hybridization (FISH) refers to the use of labeled nucleic acid sequence probes for the visualization of specific DNA or RNA sequences on mitotic chromosome preparations or in interphase cells. Since it is more sensitive compared to conventional cytogenetics, it is a method of choice for detecting specific chromosomal abnormalities in various
 
types of leukemia. Also it is used for screening and diagnosis of genetic disorders such as Down’s Syndrome (Trisomy 21) etc. in fetal specimens. Today at SRL FISH has been successfully implicated for monitoring of Her-2/neu gene amplification in breast cancer patients, for detection and quantification of chromosomal translocations i.e. BCR-ABL in CML patients, AML-ETO in AML patients and Delq for CLL patients. Olympus Microscope with Metasystem platform is used for FISH analysis.
 
 
 
RiboPrinter:
The RiboPrinter system is an automated system for identifying and/or characterizing microbial isolates (bacterial samples). The RiboPrinter system generates RiboPrint patterns based on ribosomal RNA. These patterns are used to characterize a bacterial sample, and then identify the bacterium by comparing it with an identification library established at R&D division of SRL. The RiboPrinter® system includes identification libraries with over 6400 RiboPrint™ patterns, representing over 200 bacterial genera and more than 1400 species and serotypes that are critical to the food and pharmaceutical industries. This facility is also available for researchers working on epidemiological aspects of the bacterial isolates cultured from different clinical specimens.
 
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 Benchmarks Achieved
 • Won prestigious FICCI Healthcare Excellence Award for Operational Excellence in Healthcare Delivery in 2010.
 • Received Frost & Sullivan Award in 2007 and 2009 in the recognition of being the Diagnostics Company for the respective years.
 • As of December 31, 2010, SRL, along with its subsidiary SRL Diagnostics private Limited runs eight reference laboratories, seven Centers of Excellence, 181 network laboratories (comprising 164 pathology laboratories and 17 radiology laboratories), 15 wellness centers and 888 collection centers.
Serving approximately 4.20 million customers during the nine month period ended December 31, 2010 and counting...
 
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